Membrane Protein Purification and Crystallization: A Practical Guide


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Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use.

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Details if other :. Thanks for telling us about the problem. Return to Book Page. However, obtaining high-quality protein crystals is a well known bottleneck to protein structural determination that remains a difficult task.

In this paper, it is demonstrated that recrystallization can be an effective method of improving the quality of protein crystals. Five proteins, lysozyme, proteinase K, concanavalin A, thaumatin and catalase, were used for this investigation, and the crystal quality of these proteins was examined using X-ray diffraction before and after recrystallization. Comparisons of the crystals before and after recrystallization verified that recrystallization not only enhanced the morphology of the crystals but also improved crystal quality. Therefore, it is proposed that recrystallization might be a useful alternative method for obtaining protein crystals with enhanced diffraction.

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Membrane Protein Purification and Crystallization: A Practical Guide Membrane Protein Purification and Crystallization: A Practical Guide
Membrane Protein Purification and Crystallization: A Practical Guide Membrane Protein Purification and Crystallization: A Practical Guide
Membrane Protein Purification and Crystallization: A Practical Guide Membrane Protein Purification and Crystallization: A Practical Guide
Membrane Protein Purification and Crystallization: A Practical Guide Membrane Protein Purification and Crystallization: A Practical Guide
Membrane Protein Purification and Crystallization: A Practical Guide Membrane Protein Purification and Crystallization: A Practical Guide

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